Mari- we did natural breedings.
Erik- the b*tch ovulates when the progesterone level is at 5 ng. The LH spike happens when the progesterone level is between 2 and 3. Ovulation (5 ng) usually happens about 2 days after the LH spike. Once she ovulates (drops the eggs) it takes 2 days for the eggs to mature and they stick around for 2 days once they are mature. That is why if you are doing frozen semen it is usually best to breed 3 days after ovulation. When we bred Tok using frosen semen this is what we did, and when we bred her her progesterone level was around 20 ng. We ended up getting 9 puppies. For natural breedings, we breed on either the 1st and 3rd, or the 2nd and 4th day from ovulation.